Layout of the Cell Culture Room
•Download as PPTX, PDF•
2 likes•1,256 views
Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room Layout of the Cell Culture Room
Report
Share
Report
Share
More Related Content
What's hot
Stem cell culture
Introduction
History
Stem cell characteristic
Stem Cell Differentiation
Kinds of stem cell
Embryonic stem cell
Stem cell application
Technical challenges
Conclusions
References
Cell culture, Different type of cell culture media, types of media
This document provides information on different types of cell culture media. It discusses natural media including biological fluids and tissue extracts. It also discusses artificial/synthetic media including balanced salt solutions, basal media, and complex media. Specific components of media are outlined including amino acids, vitamins, inorganic salts, glucose, serum, proteins, growth factors, hormones, and antibiotics. Different types of specialized media are also mentioned such as serum-free, chemically defined, conditioned, and protein-free media. Criteria for selecting the appropriate media for specific cell lines is discussed.
Primary and established cell line culture
Introduction
Primary Culture
Steps of Primary Culture
Isolation Of Tissue
Dissection And Disaggregation
Types Of Primary Culture
Primary Explants Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
Reference
Animal biotechnology
This document summarizes techniques for organ culture, histotypic culture, and apoptosis. It discusses how entire organs or embryos can be excised and cultured to maintain normal physiological functions and biochemical processes. Specific procedures are outlined for organ culture, including dissection, placement on supports, and incubation. Histotypic cultures allow growth of cell lines in 3D matrices to form tissue-like structures using methods like gel encapsulation, hollow fibers, or spheroid formation. Multicellular tumor spheroids are also described as a model for studying tumor cell proliferation, drug responses, and gene expression. Finally, apoptosis or programmed cell death is summarized as an orderly cell destruction process triggered via intrinsic or extrinsic pathways.
Types of animal cell culture, characterization and preservation
Animal cell culture involves growing cells outside their natural environment under controlled conditions. There are two main types of cell culture: primary cell culture which uses cells directly from an animal, and secondary cell culture which uses cell lines that can be propagated repeatedly. Cells may be adherent, attaching to culture surfaces, or in suspension. Characterization of cell lines assesses identity, purity and suitability for use. Cryopreservation allows long-term storage of cells by freezing them at very low temperatures.
Types of animal cell culture; characterization & Their preservation.
This document provides an overview of animal cell culture, including the different types (primary and secondary cell culture, cell lines), techniques used for primary culture, and characterization and preservation of animal cells. It discusses how primary cell culture involves separating cells directly from tissue and allowing them to grow under controlled conditions. Secondary cell culture refers to sub-culturing primary cells by transferring them to new vessels with fresh media. Cell lines can be propagated repeatedly and sometimes indefinitely. The document also describes cryopreservation as a method for preserving live cells and tissues at ultra-low temperatures in liquid nitrogen.
Animal cell culture (Basics)
Cultured animal cells have many important applications. They can be used as (1) model systems to study basic cell biology and interactions between cells and pathogens, (2) for toxicity testing of new drugs and chemicals, and (3) in cancer research to study normal and cancerous cell differences. Animal cell culture is also used for virology research, manufacturing of vaccines and proteins, genetic counseling, genetic engineering of cells, and gene and drug screening and development. Proper growth media, aseptic techniques, cryopreservation, and applications in various fields make animal cell culture a valuable tool.
Cell culture media
Cell culture media are designed to support the growth of cells outside their natural environment. They generally contain amino acids, salts, glucose, vitamins and other nutrients. Media can be natural (containing biological fluids) or artificial/synthetic. Artificial media are grouped into serum-containing, serum-free, chemically defined, and protein-free categories based on their ingredients. Key components of media include buffers, amino acids like glutamine, vitamins, inorganic salts, carbohydrates, proteins, lipids, trace elements, and supplements specific to cell lines. Selection of the appropriate medium depends on the cell type and purpose of culture. Primary cells especially benefit from ready-to-use conditioned media.
Stem cell culture
Stem cells can be derived from embryonic stem cells, adult stem cells, or induced pluripotent stem cells. Stem cells are undifferentiated cells that have the potential to differentiate into other cell types. There are several types of stem cells including totipotent, pluripotent, multipotent, oligopotent, and unipotent stem cells, which differ in their ability to differentiate. Stem cells offer potential for treating diseases but also raise ethical issues that require more research.
Cell synchronization
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Stem cells , culture and technologies
Stem cells have the ability to renew themselves and differentiate into specialized cell types. There are two main sources of stem cells: embryonic stem cells derived from blastocysts and adult stem cells found in adult tissues. Stem cell research aims to understand development and cell differentiation processes and develop therapies for diseases. Embryonic stem cells are pluripotent while adult stem cells are multipotent or unipotent. Stem cells are cultured in controlled conditions to maintain their undifferentiated state and are characterized based on gene expression and differentiation potential.
Bioreators ppt
1. A fluidized bed bioreactor is similar to a bubble column bioreactor but with an expanded top section.
2. In a fluidized bed bioreactor, microorganisms or cell cultures are grown while suspended in a liquid medium by the upward flow of gas or air bubbles from below.
3. This helps maintain the organisms in suspension and provides conditions for optimum growth, such as gas-liquid contact and mixing, while minimizing shear stress on the cells.
Organ culture technique in synthetic media- animal tissue culture
Organ culture is a development from tissue culture that allows for the culture of pieces of organs on artificial media to accurately model organ functions in various states. Special culture methods are required as organs require high oxygen levels. Organ pieces can be cultured on plasma clots, agar, raft methods using lens paper or rayon, grid methods, or in liquid media using supports like gauze or rafts. Organ culture faces limitations as results may not match whole animal studies due to lack of in vivo drug metabolism.
Prabhakar Singh- IV_SEM-Basic Techniques of Mammalian cell culture in vitro
Prabhakar Singh- IV_SEM-Basic Techniques of Mammalian cell culture in vitroDepartment of Biochemistry, Veer Bahadur Singh Purvanchal Univarsity, Jaunpur
This document discusses techniques for primary cell culture, including mechanical disaggregation, enzymatic disaggregation using trypsin or collagenase, and the primary explant technique. It provides details on each technique, such as how tissues are chopped or sliced, the use of enzymes at different temperatures, and factors that promote efficient development of primary cultures. The document also covers criteria for cell separation methods, cell differentiation, methods of cell transformation, and characteristics of transformed cells.Constituent of animal tissue culture media and their specific application
INTRODUCTION
HISTORY
PHYSICOCHEMICAL PROPERTIES OF CULTURE MEDIA
pH
CO2, BICARBONATE AND BUFFERING
OXYGEN
TEMPERATURE
OSMOLALITY
BALANCED SALT SOLUTIONS
CONSTITUENTS OF CULTURE MEDIA
AMINO ACIDS
VITAMINS
SALTS
GLUCOSE
OTHER ORGANIC SUPPLEMENTS
ANTIBIOTICS
SERUM
PROTEINS
NUTRIENTS AND METABOLITES
HORMONES AND GROWTH FACTORS
LIPIDS
MINERALS
INHIBITORS
APPLICATIONS OF CULTURE MEDIA
CONCLUSION
REFERENCES
Airlift fermenter
This document discusses airlift fermenters, which are a type of bioreactor. It provides three key points:
1) Airlift fermenters are pneumatic bioreactors that use gas injection and density gradients to circulate liquids without a mechanical agitator, reducing shear stress and heat generation.
2) There are two main types - internal loop fermenters with a central draft tube, and external loop fermenters with separate circulation channels.
3) Airlift fermenters are commonly used for aerobic processes, producing products like single cell proteins, due to their efficiency and ability to handle fragile cells. They have simple designs but require higher gas pressures and throughputs than stirred
Role of serum and supplements in culture medium k.s
ROLE OF SERUM AND SUPPLEMENTS IN CULTURE MEDIA
Serum is a complex mix of albumins, growth factors and growth inhibitors.
Serum is one of the most important components of cell culture media and serves as a source for amino acids, proteins, vitamins (particularly fat-soluble vitamins such as A, D, E, and K), carbohydrates, lipids, hormones, growth factors, minerals, and trace elements.
Serum from fetal and calf bovine sources are commonly used to support the growth of cells in culture.
Fetal serum is a rich source of growth factors and is appropriate for cell cloning and for the growth of fastidious cells.
Calf serum is used in contact-inhibition studies because of its lower growth-promoting properties.
Normal growth media often contain 2-10% of serum.
Supplementation of media with serum serves the following functions :
Serum provides the basic nutrients (both in the solution as well as bound to the proteins) for cells.
Serum provides several growth factors and hormones involved in growth promotion and specialized cell function.
It provides several binding proteins like albumin, transferrin, which can carry other molecules into the cell. For example: albumin carries lipids, vitamins, hormones, etc. into cells.
It also supplies proteins, like fibronectin, which promote the attachment of cells to the substrate. It also provides spreading factors that help the cells to spread out before they begin to divide.
It provides protease inhibitors which protect cells from proteolysis.
It also provides minerals, like Na+, K+, Zn2+, Fe2+, etc.
It increases the viscosity of the medium and thus, protects cells from mechanical damages during agitation of suspension cultures.
It also acts a buffer.
Due to the presence of both growth factors and inhibitors, the role of serum in cell culture is very complex.
Unfortunately, in addition to serving various functions, the use of serum in tissue culture applications has several drawbacks .
Cryopreservation
This document provides an overview of cryopreservation, which involves preserving living cells and tissues at ultra-low temperatures, typically in liquid nitrogen. It discusses the principles, processes, and steps involved, including addition of cryoprotectants, freezing and storage at temperatures below -130°C, thawing, re-culturing cells, measuring viability, and regenerating plants from cryopreserved cells or tissues. The goal is to halt biological and chemical processes to preserve living material intact for long periods of time while maintaining viability after thawing.
Primary culture and cell line
Introduction
Primary Culture
Steps In Primary Culture
Isolation Of Tissue
Dissection And/Or Disaggregation
Types Of Primary Culture
Primary Explant Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
reference
Culture media types for industrial fermentation
The document discusses different types of culture media used for industrial fermentation. There are natural/crude media which use biological sources like tissue extracts. Synthetic/artificial media use defined chemical compounds and are grouped into serum-containing, serum-free, chemically defined, and protein-free. Enrichment media add specific growth substances to basal media. Selective media contain antimicrobials or dyes to inhibit unwanted microorganisms and support growth of target organisms. Examples given are EMB agar and Mannitol Salt agar.
What's hot (20)
Cell culture, Different type of cell culture media, types of media
Cell culture, Different type of cell culture media, types of media
Types of animal cell culture, characterization and preservation
Types of animal cell culture, characterization and preservation
Types of animal cell culture; characterization & Their preservation.
Types of animal cell culture; characterization & Their preservation.
Organ culture technique in synthetic media- animal tissue culture
Organ culture technique in synthetic media- animal tissue culture
Prabhakar Singh- IV_SEM-Basic Techniques of Mammalian cell culture in vitro
Prabhakar Singh- IV_SEM-Basic Techniques of Mammalian cell culture in vitro
Constituent of animal tissue culture media and their specific application
Constituent of animal tissue culture media and their specific application
Role of serum and supplements in culture medium k.s
Role of serum and supplements in culture medium k.s
More from Zohaib HUSSAIN
Programmed Assembly of Synthetic Protocells into Thermoresponsive Prototissues
Programmed assembly of synthetic protocells into thermoresponsive prototissues
Programmed assembly of synthetic protocells into thermoresponsive prototissues
Programmed assembly of synthetic protocells into thermoresponsive prototissues
Programmed assembly of synthetic protocells into thermoresponsive prototissues
Programmed assembly of synthetic protocells into thermoresponsive prototissues
Bone Tissue Engineering
Introduction
Anatomy and Physiology of bone
Bone Tissue Engineering
Recent studies related to bone tissue engineering
Commercialized products and ongoing clinical trials
Biomedical start-ups
Concluding remarks
Introduction
Anatomy and Physiology of bone
Bone Tissue Engineering
Recent studies related to bone tissue engineering
Commercialized products and ongoing clinical trials
Biomedical start-ups
Concluding remarks
Introduction
Anatomy and Physiology of bone
Bone Tissue Engineering
Recent studies related to bone tissue engineering
Commercialized products and ongoing clinical trials
Biomedical start-ups
Concluding remarks
Large-scale Production of Stem Cells Utilizing Microcarriers
Large-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing MicrocarriersLarge-scale Production of Stem Cells Utilizing Microcarriers
Burn Dressings
Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn Dressings Burn DressingsBurn Dressings Burn Dressings
Characterization of Supramolecular Polymers
This document discusses several methods for characterizing supramolecular polymers, which are challenging to characterize due to their dynamic nature. Vapor pressure osmometry uses Raoult's law to relate vapor pressure to molecular weight. Theoretical estimation of molecular weight can be obtained from binding constants using equilibrium models. Size exclusion chromatography separates polymers by hydrodynamic radius. Viscometry uses the Mark-Houwink equation to relate intrinsic viscosity to molecular weight. Mass spectrometry, scanning probe microscopy, electron microscopy, and MALDI-TOF mass spectrometry also provide characterization of supramolecular polymers.
Translation Initiation in Eukaryotes
Translation initiation in eukaryotes is a highly regulated and rate-limiting process that involves the assembly of numerous transient complexes containing over a dozen eukaryotic initiation factors. This process culminates in the accommodation of a start codon at the appropriate ribosomal site. Structural biology has provided insights into the mammalian mitochondrial translation initiation complex and other key complexes and factors involved in the process, such as eIF3, the eIF2 ternary complex, and the DHX29 helicase. Dysregulation of translation initiation can contribute to diseases like cancer and metabolic disorders.
Mitochondrial Respiratory Complex
The document discusses mitochondrial respiratory complexes and respirasomes. It notes that the complexes assemble into larger structures called respirasomes, which are necessary for stable respiratory function. There are four main complexes involved in oxidative phosphorylation. The complexes work together to establish a proton gradient used by ATP synthase to generate ATP. Dysfunction can lead to diseases. Recent cryo-EM studies have provided structural information on respiratory supercomplexes in various organisms, revealing details of protein interactions and organization principles.
How to Convert XRD, FTIR File Data to Word Table in Order to Draw Plot on Ori...
How to Convert XRD, FTIR File Data to Word Table in Order to Draw Plot on Origin Software or Excel Spreadsheet?
Sharing is Caring
Abortion A Rising Issue In Medical Health Care Technology
Abortion
Types
Abortion legal view
Abortion ethical view
Abortion moral view
Abortion ISLAM view
Debate
Voting
PHOTOSYNTHESIS: What we have learned so far?
This document summarizes key information about photosynthesis. It discusses that photosynthesis captures light energy to convert carbon dioxide and water into glucose through chloroplasts in plant leaves. It describes the two stages of photosynthesis - the light-dependent reactions where ATP and NADPH are produced, and the Calvin cycle where glucose is produced. It also discusses C3, C4, and CAM pathways and how plants with different pathways may be impacted by increasing carbon dioxide levels. Potential targets for improving plant photosynthesis through genetic engineering or other methods are also outlined.
Industrial Production of Insulin
Contents
1. Insulin Molecule
2. Effect of Insulin in Body
3. History of Insulin
4. Recent Trends in Insulin Productions and Types
4.1 Animal Insulins
4.2 Long-Acting Insulins
4.3 Human Insulins
4.4 Insulin Analogues
4.5 Biosimilar Insulins
5. Insulin Production (Chain A and Chain B Method)
5.1 Upstream Processing
5.2 Downstream Processing
6. The Proinsulin Process
7. Insulin Available in Market with Different Brand Names
8. References
Oxidation & Reduction involves electron transfer & How enzymes find their sub...
Oxidation is loss of electrons
Reduction is gain of electrons
Oxidation is always accompanied by reduction
The total number of electrons is kept constant
Oxidizing agents oxidize and are themselves reduced
Reducing agents reduce and are themselves oxidized
Cellulase (Types, Sources, Mode of Action & Applications)
Cellulase is an enzyme system consisting of endo- and exo-glucanases and cellobiase that catalyzes the hydrolysis of cellulose. There are three major types of cellulases - endoglucanase, exoglucanase, and beta-glucosidase. Cellulase-producing microbes employ one of three mechanisms: free cellulase systems using individual enzymes, cellulosome complexes, or endoglucanases without other domains. The synergistic action of endo- and exoglucanases supplemented by beta-glucosidase completely degrades cellulose to glucose. Cellulases find applications in food, animal feed, textiles, biofu
Amylases (Types, Sources, Mode of Action & Applications)
Amylases are important hydrolase enzymes which have been widely used since many decades. These enzymes randomly cleave internal glycosidic linkages in starch molecules to hydrolyze them and yield dextrins and oligosaccharides. Among amylases α-Amylase is in maximum demand due to its wide range of applications in the industrial front. α-Amylase can be produced by plant or microbial sources. The ubiquitous nature, ease of production and broad spectrum of applications make α-Amylase an industrially important enzyme.
Life on Earth (By Alonso Ricardo and Jack W. Szostak) Summary (By Zohaib Hus...
Life on Earth (By Alonso Ricardo and Jack W. Szostak)
Summary (By Zohaib Hussain)
Life on Earth (By Alonso Ricardo and Jack W. Szostak)
Summary (By Zohaib Hussain)
Life on Earth (By Alonso Ricardo and Jack W. Szostak)
Summary (By Zohaib Hussain)
Life on Earth (By Alonso Ricardo and Jack W. Szostak)
Summary (By Zohaib Hussain)
Eukaryotic Gene Regulation: Detailed Review
1. Levels of gene regulation
The observation that differences in the RNA and protein content of different tissues are not paralleled by significant differences in their DNA content indicates that the process whereby DNA produces mRNA must be the level at which gene expression is regulated in eukaryotes. In bacteria this process involves only a single stage, that of transcription, in which RNA copy of the DNA is produced by the enzyme RNA polymerase. Even while this process is still occurring, ribosomes attach to the nascent RNA chain and begin to translate it into protein. Hence cases
of gene regulation in bacteria, such as the switching on of the synthesis of the enzyme β-galactosidase in response to the presence of lactose (its substrate), are mediated by increased transcription of the appropriate gene. Clearly, a similar regulation of gene transcription in different tissues, or in response to substances such as steroid hormones which induce the synthesis of new proteins, represents an attractive method of gene regulation in eukaryotes.
In contrast to the situation in bacteria, however, a number of stages intervene between the initial synthesis of the primary RNA transcript and the eventual production of mRNA (Fig. 1).
The initial transcript is modified at its 5′ end by the addition of a cap structure containing a modified guanosine residue and is subsequently cleaved near its 3′ end, followed by the addition of up to 200 adenosine residues in a process known as polyadenylation. Subsequently, intervening sequences or introns, which interrupt the protein-coding sequence in both the DNA and the primary transcript of many genes. Although this produces a functional mRNA, the spliced molecule must then be transported from the nucleus, where these processes occur, to the cytoplasm where it can be translated into protein.
Telomere, Functions & Role in Aging & Cancer
Telomeres cap the ends of chromosomes and protect them from degradation during cell division. As cells divide, telomeres shorten due to the inability of DNA replication enzymes to fully copy chromosome ends. This limits a cell to around 50-70 divisions before entering senescence. Cancer cells activate telomerase to maintain telomere length, allowing unlimited division. Telomeres play a key role in both aging and cancer - their shortening limits the lifespan of normal cells but cancer cells overcome this via telomerase to achieve immortality and uncontrolled growth. Measuring and targeting telomerase may provide new strategies for cancer detection and treatment.
Eukaryotic and Prokaryotic Chromosomes
Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes
Chromosome: A Complete Overview
Chromosomes are bundles of tightly coiled DNA located within the nucleus of almost every cell in our body. A chromosome is a DNA molecule with part or all of the genetic material (genome) of an organism. Chromosomes are normally visible under a light microscope only when the cell is undergoing the metaphase of cell division. Before this happens, every chromosome is copied once (S phase), and the copy is joined to the original by a centromere, resulting in an X-shaped structure. The original chromosome and the copy are now called sister chromatids. During metaphase, when a chromosome is in its most condensed state, the X-shape structure is called a metaphase chromosome.
Chemical Modifications of Protein and its Applications
This document discusses chemical modifications of proteins. Chemical modifications can be done intentionally to study structure-function relationships, develop new products, or improve existing ones. Modifications are used in fields like pharmacology, food production, and industry. Specific modifications discussed include sulfhydryl and disulfide modifications by oxidation, and reduction of disulfide bonds. Applications mentioned are in basic protein chemistry, food and nutrition to prevent deterioration or alter properties, and pharmaceuticals to alter biological properties, target drug delivery, or influence drug lifetime in the body.
More from Zohaib HUSSAIN (20)
Programmed Assembly of Synthetic Protocells into Thermoresponsive Prototissues
Programmed Assembly of Synthetic Protocells into Thermoresponsive Prototissues
Large-scale Production of Stem Cells Utilizing Microcarriers
Large-scale Production of Stem Cells Utilizing Microcarriers
How to Convert XRD, FTIR File Data to Word Table in Order to Draw Plot on Ori...
How to Convert XRD, FTIR File Data to Word Table in Order to Draw Plot on Ori...
Abortion A Rising Issue In Medical Health Care Technology
Abortion A Rising Issue In Medical Health Care Technology
Oxidation & Reduction involves electron transfer & How enzymes find their sub...
Oxidation & Reduction involves electron transfer & How enzymes find their sub...
Cellulase (Types, Sources, Mode of Action & Applications)
Cellulase (Types, Sources, Mode of Action & Applications)
Amylases (Types, Sources, Mode of Action & Applications)
Amylases (Types, Sources, Mode of Action & Applications)
Life on Earth (By Alonso Ricardo and Jack W. Szostak) Summary (By Zohaib Hus...
Life on Earth (By Alonso Ricardo and Jack W. Szostak) Summary (By Zohaib Hus...
Chemical Modifications of Protein and its Applications
Chemical Modifications of Protein and its Applications
Recently uploaded
Science grade 09 Lesson1-2 NLC-pptx.pptx
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
just download it to see!
ThrombUS+ Project Presentation - June 2024
Kaldoudi E., ThrombUS+ Project Overview, Horizon Europe Health Cluster Networking Meeting, Online Event, 26 June 2024
Electrostatic force class 8 ncert. .pptx
This an presentation about electrostatic force. This topic is from class 8 Force and Pressure lesson from ncert . I think this might be helpful for you. In this presentation there are 4 content they are Introduction, types, examples and demonstration. The demonstration should be done by yourself
The extremotolerant desert moss Syntrichia caninervis is a promising pioneer ...
Many plans to establish human settlements on other planets focus on
adapting crops to growth in controlled environments. However, these settlements will also require pioneer plants that can grow in the soils and
harsh conditions found in extraterrestrial environments, such as those
on Mars. Here, we report the extraordinary environmental resilience of Syntrichia caninervis, a desert moss that thrives in various extreme environments. S. caninervis has remarkable desiccation tolerance; even after
losing >98% of its cellular water content, it can recover photosynthetic
and physiological activities within seconds after rehydration. Intact plants
can tolerate ultra-low temperatures and regenerate even after being stored
in a freezer at 80C for 5 years or in liquid nitrogen for 1 month.
S. caninervis also has super-resistance to gamma irradiation and can survive and maintain vitality in simulated Mars conditions; i.e., when simultaneously exposed to an anoxic atmosphere, extreme desiccation, low temperatures, and intense UV radiation. Our study shows that S. caninervis is
among the most stress tolerant organisms. This work provides fundamental insights into the multi-stress tolerance of the desert moss
S. caninervis, a promising candidate pioneer plant for colonizing extraterrestrial environments, laying the foundation for building biologically sustainable human habitats beyond Earth.
The National Research Platform Enables a Growing Diversity of Users and Appl...
Remote Presentation
Emerging Centers Track
Campus Research Computing Consortium (CaRCC)
June 21, 2023
Towards Wearable Continuous Point-of-Care Monitoring for Deep Vein Thrombosis...
Kaldoudi E, Marozas M, Jurkonis R, Pousset N, Legros M, Kircher M, Novikov D, Sakalauskas A, Moustakidis P, Ayinde B, Moltani LA, Balling S, Vehkaoja A, Oksala N, Macas A, Balciuniene N, Bigaki M, Potoupnis M, Papadopoulou S-L, Grandone E, Gautier M, Bouda S, Schloetelburg C, Prinz T, Dionisio P, Anagnostopoulos S, Drougka I, Folkvord F, Drosatos G, Didaskalou S and the ThrombUS+ Consortium, Towards Wearable Continuous Point-of-Care Monitoring for Deep Vein Thrombosis of the Lower Limb. In: Jarm, T., Šmerc, R., Mahnič-Kalamiza, S. (eds) 9th European Medical and Biological Engineering Conference. EMBEC 2024. IFMBE Proceedings, vol 113. Springer, Cham. https://doi.org/10.1007/978-3-031-61628-0_36
Presented by Dr. Stelios Didaskalou, ThrombUS+ Project Manager
What is data analytics ,Data science,Data processing chain,regression,decisio...
about Data analytics.
一比一原版(wsu文凭证书)美国华盛顿州立大学毕业证如何办理
原版一模一样【微信：741003700 】【(wsu文凭证书)美国华盛顿州立大学毕业证成绩单】【微信：741003700 】学位证，留信学历认证（真实可查，永久存档）原件一模一样纸张工艺/offer、在读证明、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
办理(wsu文凭证书)美国华盛顿州立大学毕业证【微信：741003700 】外观非常简单，由纸质材料制成，上面印有校徽、校名、毕业生姓名、专业等信息。
办理(wsu文凭证书)美国华盛顿州立大学毕业证【微信：741003700 】格式相对统一，各专业都有相应的模板。通常包括以下部分：
校徽：象征着学校的荣誉和传承。
校名:学校英文全称
授予学位：本部分将注明获得的具体学位名称。
毕业生姓名：这是最重要的信息之一，标志着该证书是由特定人员获得的。
颁发日期：这是毕业正式生效的时间，也代表着毕业生学业的结束。
其他信息：根据不同的专业和学位，可能会有一些特定的信息或章节。
办理(wsu文凭证书)美国华盛顿州立大学毕业证【微信：741003700 】价值很高，需要妥善保管。一般来说，应放置在安全、干燥、防潮的地方，避免长时间暴露在阳光下。如需使用，最好使用复印件而不是原件，以免丢失。
综上所述，办理(wsu文凭证书)美国华盛顿州立大学毕业证【微信：741003700 】是证明身份和学历的高价值文件。外观简单庄重，格式统一，包括重要的个人信息和发布日期。对持有人来说，妥善保管是非常重要的。
Principles of Colorimetry - Mastering the Art of Colour Measurement
Unlock the secrets of colour science with our comprehensive presentation on the principles of colorimetry. This guide delves into the critical aspects of colour measurement, essential for ensuring consistent quality and customer satisfaction across various industries.
Key Topics Covered:
- Introduction to Colorimetry
- Human Perception of Colour
- Importance of Metamerism and Colour Constancy
- Understanding Different Colour Spaces (RGB, CMYK, Lab)
Utilising Spectrophotometers and Colorimeters
- Implementing Quality Control for Colour Consistency
Learn how mastering these techniques can enhance product quality and meet customer expectations. Perfect for professionals in printing, textiles, paint, food, cosmetics, automotive, electronics, and more.
Read the full article: https://colours.guide/principles-of-colorimetry/
Visit www.colours.guide for more insights and resources on colour science.
ThrombUS+ Project Overview - January 2024
E. Kaldoudi, ThrombUS+ Project Inaugural Presentation, Project Meeting #01, ThrombUS+ Horizon Innovation Action No. 101137227, Alexandroupoli, Greece, 24 January 2024
ScieNCE grade 08 Lesson 1 and 2 NLC.pptx
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it................
just download it..............
@Call @Girls in Nagpur 🤷♂️ XXXXXXXX 🤷♂️Tanisha Sharma Best High Class Nag...
For Ad Post Contact :- adityaroy0215@gmail.com
TOPIC: INTRODUCTION TO FORENSIC SCIENCE.pptx
This presentation, "Introduction to Forensic Science," offers a basic understanding of forensic science, including its history, why it's needed, and its main goals. It covers how forensic science helps solve crimes and its importance in the justice system. By the end, you'll have a clear idea of what forensic science is and why it's essential.
SPERM DNA DAMAGE/SPERM DNA FRAGMENTATION.pptx
SPERM DFI, SPERM DNA DAMAGE,
TEST FOR DFI, CLINICAL IMPLICATIONS OF DFI
PART 1 The New Natural Principles of Electromagnetism and Electromagnetic Fie...
Document Summary and the History of Perpetual Motion
Every single Faraday Generator coil since 1834 has been and is currently performing Negative Work at infinite efficiency with created Electromagnetic Field Energy during electricity generation and its physical Kinetic Energy reduction or Electromagnetic Resistance of the changing magnetic field which is initially inducing Electric Current in the generator coil according to Faraday's Law of Induction.
The Work-Energy Principle confirms mathematically that the magnitude of the changing magnetic field's Kinetic Energy reduction is equal to the magnitude of Negative Work performed at infinite efficiency, which is equal to the magnitude of Energy (Electromagnetic Field Energy which is created according to Oersted's Law of Creation of Energy of 1820). Created Electromagnetic Field Energy is required in order to perform the Negative Work – because Work cannot be performed in the absence of Energy.
In 2007 Thane Heins of Almonte Ontario, Canada discovered that unlimited amounts of Positive Electromechanical Work could be performed at infinite efficiency with created and TIME DELAYED Electromagnetic Field Energy.
Every single ReGenX Generator coil since 2007 has been and is currently performing Positive Work at infinite efficiency with created Electromagnetic Field Energy during electricity generation and during its physical Kinetic Energy increase or Electromagnetic Assistance of the changing magnetic field which is initially inducing Electric Current in the generator coil according to Heins' Law of Induction.
Faraday Electric Generators all harness internally Created Electromagnetic Field Energy in order to perform Negative Work (system Kinetic Energy reduction) at infinite efficiency and ReGenX Electric Generators harness internally created and Time Delayed Electromagnetic Field Energy in order to perform Positive Work (system Kinetic Energy increase) at infinite efficiency.
Both Faraday Generators and ReGenX Generators operate as Perpetual Motion Machines of the First Kind because they both have the ability to perform both Negative or Positive Work indefinitely and at infinite efficiency without requiring any External Energy input. The unlimited Energy required to perform either the Negative or Positive Work is created at the Sub-Atomic Quantum Electron level inside the generators' Current Bearing Wires according to the Law of Creation of Energy.
Hans Christian Oersted discovered the Law of Creation of Energy in 1820 when he demonstrated the world's first Perpetual Motion Machine of the First Kind at the University of Copenhagen when he also simultaneously violated Newton's 1st, 2nd and 3rd Laws of Motion.
Michael Faraday built and demonstrated the world's second Perpetual Motion Machine of the First Kind in 1822 when he demonstrated his Electric Motor invention which harnessed created Electromagnetic Field Energy in order to perform Positive Electromechanical Work at infinite efficienc
ALTERNATIVE ANIMAL TOXICITY STUDY .pptx
Alternatives animal testing are development and implementation of test methods that avoid the use of live animals.
Human biochemistry, physiology, pharmacology, and endocrinology and toxicology has been derived from animal models.10-100 millions of animals are using for experimentation in a year.
Animals used experimentation distributed among zebra- fish to primates.
Vast majority of animals are sacrificed at end of research programme.The use of animals can be further subdivided according to the degree of suffering
Minor animal suffering:- observing animals in behavioral studies, single blood sampling, Immunization without adjutants, etc.
Moderate animal suffering:- repeated blood sampling, recovery from general anesthesia, etc.
Cause and solution of Water hyacinth (Terror of Bengal)
Water hyacinth is a buoyant plant that has a clump of leaves with squishy stalks arising from the lower feathery roots and it is an invasive species came from South America
Recently uploaded (20)
The extremotolerant desert moss Syntrichia caninervis is a promising pioneer ...
The extremotolerant desert moss Syntrichia caninervis is a promising pioneer ...
The National Research Platform Enables a Growing Diversity of Users and Appl...
The National Research Platform Enables a Growing Diversity of Users and Appl...
Towards Wearable Continuous Point-of-Care Monitoring for Deep Vein Thrombosis...
Towards Wearable Continuous Point-of-Care Monitoring for Deep Vein Thrombosis...
What is data analytics ,Data science,Data processing chain,regression,decisio...
What is data analytics ,Data science,Data processing chain,regression,decisio...
Principles of Colorimetry - Mastering the Art of Colour Measurement
Principles of Colorimetry - Mastering the Art of Colour Measurement
@Call @Girls in Nagpur 🤷♂️ XXXXXXXX 🤷♂️Tanisha Sharma Best High Class Nag...
@Call @Girls in Nagpur 🤷♂️ XXXXXXXX 🤷♂️Tanisha Sharma Best High Class Nag...
PART 1 The New Natural Principles of Electromagnetism and Electromagnetic Fie...
PART 1 The New Natural Principles of Electromagnetism and Electromagnetic Fie...
Cause and solution of Water hyacinth (Terror of Bengal)
Cause and solution of Water hyacinth (Terror of Bengal)
Layout of the Cell Culture Room
- 1. BS Level 1 BS Level 3 13 feet 13 feet 3.0 feet LabCoat area 4.5 feet SINK Shoes Fridge Liquid Nitrogen Water Bath Laminar Flow Hood C02 Incubator Centrifuge Machine CO2 Tank Slide Entrance Slide Entrance Door Sterilization Zone Chemicals & Glassware's Cupboard Microscope BS Level 2 *BSL Stands for Biosafety Levels LayoutoftheCellCultureRoom Note: All measurements are done in scale and are accurate 6.5 feet