Single-molecule studies can overcome the complications of asynchrony and ensemble-
averaging in bulk-phase measurements, provide mechanistic insights into molecular …

RecBCD enzyme is a processive DNA helicase and nuclease that participates in the repair
of chromosomal DNA through homologous recombination,. We have visualized directly the …

RecBCD is a multifunctional enzyme that possesses both helicase and nuclease activities.
To gain insight into the mechanism of its helicase function, RecBCD unwinding at low …

In physiological settings, nucleic-acid translocases must act on substrates occupied by other
proteins, and an increasingly appreciated role of translocases is to catalyse protein …

Escherichia coli RecBCD is a heterotrimeric helicase/nuclease that catalyses a complex
reaction in which double-strand breaks in DNA are processed for repair by homologous …

Major pathways of recombinational DNA repair in Escherichia coli require the RecBCD
protein—a heterotrimeric, ATP-driven, DNA translocating motor enzyme. RecBCD combines …

RecBCD is a multi-functional enzyme complex that processes DNA ends resulting from a
double-strand break. RecBCD is a bipolar helicase that splits the duplex into its component …

Helicases are molecular motors that move along and unwind double-stranded nucleic acids.
RecBCD enzyme is a complex helicase and nuclease, essential for the major pathway of …

DNA helicases are a class of molecular motors that catalyze processive unwinding of double
stranded DNA. In spite of much study, we know relatively little about the mechanisms by …

We have developed and optimized a stopped-flow fluorescence assay for use in studying
DNA unwinding catalyzed by Escherichia coli RecBCD helicase. This assay monitors …