Identical sequences, different behaviors: Protein diversity captured at the single-molecule level
R Tapia-Rojo, A Alonso-Caballero, CL Badilla… - Biophysical …, 2024 - cell.com
The classical" one sequence, one structure, one function" paradigm has shaped much of our
intuition of how proteins work inside the cell. Partially due to the insight provided by bulk …
intuition of how proteins work inside the cell. Partially due to the insight provided by bulk …
Synergy between RecBCD subunits is essential for efficient DNA unwinding
R Zananiri, O Malik, S Rudnizky, V Gaydar… - Elife, 2019 - elifesciences.org
The subunits of the bacterial RecBCD act in coordination, rapidly and processively
unwinding DNA at the site of a double strand break. RecBCD is able to displace DNA …
unwinding DNA at the site of a double strand break. RecBCD is able to displace DNA …
Nanocaged enzymes with enhanced catalytic activity and increased stability
US10669534B2 - Nanocaged enzymes with enhanced catalytic activity and increased stability
- Google Patents US10669534B2 - Nanocaged enzymes with enhanced catalytic activity and …
- Google Patents US10669534B2 - Nanocaged enzymes with enhanced catalytic activity and …
Fine-tuning of RecBCD expression by post-transcriptional regulation is required for optimal DNA repair in Escherichia coli
I Kalita, IA Iosub, S Granneman, ME Karoui - bioRxiv, 2021 - biorxiv.org
To preserve genome integrity, all living organisms have developed strategies to respond to
chromosomal damage. One such response is the repair of DNA double-strand breaks …
chromosomal damage. One such response is the repair of DNA double-strand breaks …
Recombination hotspots attenuate the coupled ATPase and translocase activities of an AddAB-type helicase–nuclease
NS Gilhooly, MS Dillingham - Nucleic acids research, 2014 - academic.oup.com
In all domains of life, the resection of double-stranded DNA breaks to form long 3′-ssDNA
overhangs in preparation for recombinational repair is catalyzed by the coordinated …
overhangs in preparation for recombinational repair is catalyzed by the coordinated …
Single molecule approaches to monitor the recognition and resection of double-stranded DNA breaks during homologous recombination
The fate of a cell depends on its ability to repair the many double-stranded DNA breaks
(DSBs) that occur during normal metabolism. Improper DSB repair may result in genomic …
(DSBs) that occur during normal metabolism. Improper DSB repair may result in genomic …
Measuring two at the same time: combining magnetic tweezers with single-molecule FRET
M Swoboda, MS Grieb, S Hahn, M Schlierf - Fluorescent methods for …, 2014 - Springer
Molecular machines are the workhorses of the cell that efficiently convert chemical energy
into mechanical motion through conformational changes. They can be considered powerful …
into mechanical motion through conformational changes. They can be considered powerful …
E. coli RecBCD Nuclease Domain Regulates Helicase Activity but not Single Stranded DNA Translocase Activity
Much is still unknown about the mechanisms by which helicases unwind duplex DNA.
Whereas structure-based models describe DNA unwinding as a consequence of …
Whereas structure-based models describe DNA unwinding as a consequence of …
A chaotic dynamical system that paints and samples
T Sahai, G Mathew, A Surana - IFAC-PapersOnLine, 2017 - Elsevier
Can a dynamical system paint masterpieces such as Da Vinci's Mona Lisa or Monet's Water
Lilies? Moreover, can this dynamical system be chaotic in the sense that although the …
Lilies? Moreover, can this dynamical system be chaotic in the sense that although the …
MRN and Topoisomerase IIIα-RMI1/2 synchronize DNA resection motor proteins
DNA resection—the nucleolytic processing of broken DNA ends—is the first step of
homologous recombination. Resection is catalyzed by the resectosome, a multi-enzyme …
homologous recombination. Resection is catalyzed by the resectosome, a multi-enzyme …