Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …

Single-molecule observation and manipulation have come of age. With the advent of optical
tweezers and other methods for probing and imaging single molecules, investigators have …

In the last decades, optical tweezers have progressively emerged as a unique tool to
investigate the biophysical world, allowing to manipulate and control forces and movements …

Single molecule force spectroscopy methods, such as optical and magnetic tweezers and
atomic force microscopy, have opened up the possibility to study biological processes …

Over the past two decades, single-molecule techniques have evolved into robust tools to
study many fundamental biological processes. The combination of optical tweezers with …

Over the last decade, single-molecule techniques have proven their wide applicability in the
study of processive motor proteins and other enzymes, yielding insight into their kinetics and …

Single-molecule techniques are powerful tools that can be used to study the kinetics and
mechanics of a variety of enzymes and their complexes. Force spectroscopy, for example …

Highlights•Optical tweezers provide a sensitive tool to probe structural dynamics.•Native and
non-native folding can be characterized in detail.•Effects of intermolecular interactions on …

Cellular DNA is continuously engaged in a molecular dance with many accessory proteins.
These proteins are responsible for orchestrating the storage, maintenance, and transfer of …

Combining optical tweezers with single molecule fluorescence offers a powerful technique
to study the biophysical properties of single proteins and molecules. However, such …