… Here we discuss recent advances made using optical tweezers to study topics such as intra-…
The principles and construction of optical tweezers are reviewed in detail elsewhere; with …

… , such as atomic force microscopy and optical tweezers, were used to study the elastic
properties and mechanically induced structural transitions of nucleic acids at the single-molecule …

… consist of immobilizing and subjecting a nucleic acid construct to tension. By … nucleic acid
binding thermodynamics and kinetics and to determine the mechanism of protein–nucleic acid …

… This description is followed by a survey of applications of these methods to the studies of
protein–nucleic acid interactions, protein/RNA folding and molecular motors. We also discuss …

… The former developments have been motivated by the desire to understand conformational
changes in proteins and nucleic acids at their most fundamental spatial and temporal scales. …

… optical tweezers and their application to nucleic acid processing proteins. We first review
the basic principles of optical tweezers … of protein-nucleic acid interactions in representative …

… nucleic acids such as DNA replication and repair, as well as RNA transcription and translation,
it is important to understand the behavior of nucleic acids … properties of nucleic acids that …

… This setup permits simultaneous spatial sampling and high-resolution force measurements
of nucleic acids and proteins. First, we demonstrate the rapid spatial localization of …

… Neuman and Nagy 22 compared the capabilities and limitations of optical tweezers, magnetic
tweezers, and AFM in the context single-molecule force spectroscopy. Gross 23 described …

… fashion for packaging the viral nucleic acid genome. Nucleic acids, whose elasticity and …
, are also amenable to examination with optical tweezers. In this chapter, we provide a …