Understanding how the genome is structurally organized as chromatin is essential for
understanding its function. Here, we review recent developments that allowed the …

Gene regulation in eukaryotes requires the controlled access of sequence-specific
transcription factors (TFs) to their sites in a chromatin landscape dominated by …

The spatial organization of chromatin critically affects genome function. Recent chromosome-
conformation-capture studies have revealed topologically associating domains (TADs) as a …

Gene regulation is chiefly determined at the level of individual linear chromatin molecules,
yet our current understanding of cis-regulatory architectures derives from fragmented …

The classic view of nucleosome organization at active promoters is that two well-positioned
nucleosomes flank a nucleosome-depleted region (NDR). However, this view has been …

Micrococcal nuclease (MNase) is widely used to map nucleosomes. However, its aggressive
endo-/exo-nuclease activities make MNase-seq unreliable for determining nucleosome …

Nucleosome organization influences gene activity by controlling DNA accessibility to
transcription machinery. Here, we develop a chemical biology approach to determine …

We have developed a chemical cleavage method that allows precise mapping of both single
nucleosomes and linkers genome-wide in budding yeast. We confirm that S. cerevisiae …

Micrococcal nuclease (MNase) is commonly used to map nucleosomes genome-wide, but
nucleosome maps are affected by the degree of digestion. It has been proposed that many …

Transcriptome sequencing is a powerful technique to study molecular changes that underlie
the differences in physiological conditions and disease progression. A typical question that …