Gene regulation in eukaryotes requires the controlled access of sequence-specific
transcription factors (TFs) to their sites in a chromatin landscape dominated by …

The spatial organization of chromatin critically affects genome function. Recent chromosome-
conformation-capture studies have revealed topologically associating domains (TADs) as a …

The compact structure of the nucleosome limits DNA accessibility and inhibits the binding of
most sequence-specific proteins. Nucleosomes are not randomly located on the DNA but …

We determined genome-wide nucleosome occupancies in mouse embryonic stem cells and
their neural progenitor and embryonic fibroblast counterparts to assess features associated …

We have developed a chemical cleavage method that allows precise mapping of both single
nucleosomes and linkers genome-wide in budding yeast. We confirm that S. cerevisiae …

Micrococcal nuclease (MNase) is commonly used to map nucleosomes genome-wide, but
nucleosome maps are affected by the degree of digestion. It has been proposed that many …

Nucleosomal DNA is thought to be generally inaccessible to DNA-binding factors, such as
micrococcal nuclease (MNase). Here, we digest Drosophila chromatin with high and low …

RSC and SWI/SNF are related ATP-dependent chromatin remodeling machines that move
nucleosomes, regulating access to DNA. We addressed their roles in nucleosome phasing …

The nucleosome repeat length (NRL) is an integral chromatin property important for its
biological functions. Recent experiments revealed several conflicting trends of the NRL …

Nucleosome is a histone–DNA complex known as the fundamental repeating unit of
chromatin. Up to 90% of eukaryotic DNA is wrapped around consecutive octamers made of …