Resolution (chromatography): Difference between revisions
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:<math>R_s = \cfrac{t_{R2}-t_{R1}}{w_b} </math>. |
:<math>R_s = \cfrac{t_{R2}-t_{R1}}{w_b} </math>. |
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==Plate number== |
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[[File:Rt 5 15.png|thumb|Two resolved peaks in a chromatogram]] |
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The [[theoretical plate]] height is given by |
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:<math>H = \frac{L}{N} </math> |
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where L is the column length and N the number of theoretical plates.<ref>{{GoldBookRef | title = plate number, N | file = P04694}}</ref> The relation between plate number and peak width at the base is given by |
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:<math>N = 16 \cdot \left(\frac{t_R}{W_b}\right)^2 \,</math>. |
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==See also== |
==See also== |
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*[[Van Deemter equation]] |
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*[[Resolution (mass spectrometry)]] |
*[[Resolution (mass spectrometry)]] |
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*[[Resolution (electron density)]] |
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*[[Image resolution]] |
*[[Image resolution]] |
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Revision as of 20:42, 23 March 2019
In chromatography, resolution is a measure of the separation of two peaks of different retention time ΔM, in a chromatogram.[1][2]
Expression
Chromatographic peak resolution is given by
where rR is the retention time and wb is the peak width at baseline. Here compound 2 elutes before compound 1.
If the peaks have the same width
- .
Plate number
The theoretical plate height is given by
where L is the column length and N the number of theoretical plates.[3] The relation between plate number and peak width at the base is given by
- .
See also
References
- ^ IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "resolution in mass spectroscopy". doi:10.1351/goldbook.P04465
- ^ Ettre, L. S. (1993). "Nomenclature for chromatography (IUPAC Recommendations 1993)". Pure and Applied Chemistry. 65 (4): 819–872. doi:10.1351/pac199365040819. ISSN 1365-3075.
- ^ IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "plate number, N". doi:10.1351/goldbook.P04694
External links
Wikimedia Commons has media related to Chromatography.