Microraft: Difference between revisions
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[[File:Culture of cells on 1009F and PS-AA microraft arrays.png|thumb|Culture of cells on 1009F and PS-AA microraft arrays.]] |
[[File:Culture of cells on 1009F and PS-AA microraft arrays.png|thumb|Culture of cells on 1009F and PS-AA microraft arrays.]] |
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A '''Microraft''' ('''Isoraft''') is an arrays of microwells for [[cell sorting]], [[Cell culture#Isolation of cells|isolating cells]], analyzing [[Cell (biology)|cells]] over time, and generating [[Clonal colony|clonal populations]].<ref name="WangPhillips2010">{{cite journal|last1=Wang|first1=Yuli|last2=Phillips|first2=Colleen|last3=Xu|first3=Wei|last4=Pai|first4=Jeng-Hao|last5=Dhopeshwarkar|first5=Rahul|last6=Sims|first6=Christopher E.|last7=Allbritton|first7=Nancy|title=Micromolded arrays for separation of adherent cells|journal=Lab on a Chip|volume=10|issue=21|year=2010|pages=2917|issn=1473-0197|doi=10.1039/c0lc00186d}}</ref> This platform provides [[biomedical scientist]]s with access to diverse [[cell culture]] surfaces with integrated, easy-to-use cell separating capabilities at low cost. |
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==Platform |
==Platform== |
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The microrafts have bases composed of detachable [[wikt:Special:Search/concave|concave]] elements fabricated by a [[dip-coating]] process using a [[polydimethylsiloxane]] mold as the template and the array substrate. This manufacturing approach allows the microrafts to possess low [[autofluorescence]] and can therefore be utilized for fluorescence-based identification of cells. Cells plated on the microarray settle and attach at the center of the wells due to the microrafts’ concavity. Individual microrafts are dislodged using a needle inserted through the compliant [[polymer]] substrate. The hard polymer material of the microrafts protect the cells from damage by the needle. Cell analysis and isolation can be carried out using a standard [[inverted microscope]]. Released cells/microrafts can be collected, cultured and clonally expanded.<ref name="PMC2994190">{{cite journal |
The microrafts have bases composed of detachable [[wikt:Special:Search/concave|concave]] elements fabricated by a [[dip-coating]] process using a [[polydimethylsiloxane]] mold as the template and the array substrate. This manufacturing approach allows the microrafts to possess low [[autofluorescence]] and can therefore be utilized for fluorescence-based identification of cells. Cells plated on the microarray settle and attach at the center of the wells due to the microrafts’ concavity. Individual microrafts are dislodged using a needle inserted through the compliant [[polymer]] substrate. The hard polymer material of the microrafts protect the cells from damage by the needle. Cell analysis and isolation can be carried out using a standard [[inverted microscope]]. Released cells/microrafts can be collected, cultured and clonally expanded.<ref name="PMC2994190">{{cite journal|author=Wang Y|title=Micromolded Arrays for Separation of Adherent Cells|journal=Lab Chip|volume=10|issue=21|pages=2917–2924|date=November 2011|pmid=20838672|doi=10.1039/c0lc00186d|last2=Phillips|first2=C|last3=Xu|first3=W|last4=Pai|first4=JH|last5=Dhopeshwarkar|first5=R|last6=Sims|first6=CE|last7=Allbritton|first7=N|pmc=2994190 }}</ref><ref name="PMC3194786">{{cite journal|author=Gach PC|title=Isolation and manipulation of living adherent cells by micromolded magnetic rafts|journal=Biomicrofluidics|volume=5|issue=3|pages=32002–32012|date=September 2011|pmid=22007266|doi=10.1063/1.3608133|last2=Wang|first2=Y|last3=Phillips|first3=C|last4=Sims|first4=CE|last5=Allbritton|first5=NL|pmc=3194786 }}</ref> |
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==Utility== |
==Utility== |
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Using this system, extremely high single-cell cloning rates of greater than 95% have been achieved. |
Using this system, extremely high single-cell cloning rates of greater than 95% have been achieved.{{cn}} |
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This system is ideal for both [[subculture (biology)#Adherent cells|adherent]] and [[subculture (biology)#Non-adherent cells|non-adherent]] cell types. |
This system is ideal for both [[subculture (biology)#Adherent cells|adherent]] and [[subculture (biology)#Non-adherent cells|non-adherent]] cell types. |
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==References== |
==References== |
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{{reflist}} |
{{reflist}} |
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[[Category:Cells]] |
[[Category:Cells]] |
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Revision as of 22:51, 29 January 2019
 | This article includes a list of general references, but it lacks sufficient corresponding inline citations. (June 2013) |
A Microraft (Isoraft) is an arrays of microwells for cell sorting, isolating cells, analyzing cells over time, and generating clonal populations.[1] This platform provides biomedical scientists with access to diverse cell culture surfaces with integrated, easy-to-use cell separating capabilities at low cost.
Platform
The microrafts have bases composed of detachable concave elements fabricated by a dip-coating process using a polydimethylsiloxane mold as the template and the array substrate. This manufacturing approach allows the microrafts to possess low autofluorescence and can therefore be utilized for fluorescence-based identification of cells. Cells plated on the microarray settle and attach at the center of the wells due to the microrafts’ concavity. Individual microrafts are dislodged using a needle inserted through the compliant polymer substrate. The hard polymer material of the microrafts protect the cells from damage by the needle. Cell analysis and isolation can be carried out using a standard inverted microscope. Released cells/microrafts can be collected, cultured and clonally expanded.[2][3]
Utility
Using this system, extremely high single-cell cloning rates of greater than 95% have been achieved.[citation needed]
This system is ideal for both adherent and non-adherent cell types.
References
- ^ Wang, Yuli; Phillips, Colleen; Xu, Wei; Pai, Jeng-Hao; Dhopeshwarkar, Rahul; Sims, Christopher E.; Allbritton, Nancy (2010). "Micromolded arrays for separation of adherent cells". Lab on a Chip. 10 (21): 2917. doi:10.1039/c0lc00186d. ISSN 1473-0197.
- ^ Wang Y; Phillips, C; Xu, W; Pai, JH; Dhopeshwarkar, R; Sims, CE; Allbritton, N (November 2011). "Micromolded Arrays for Separation of Adherent Cells". Lab Chip. 10 (21): 2917–2924. doi:10.1039/c0lc00186d. PMC 2994190. PMID 20838672.
- ^ Gach PC; Wang, Y; Phillips, C; Sims, CE; Allbritton, NL (September 2011). "Isolation and manipulation of living adherent cells by micromolded magnetic rafts". Biomicrofluidics. 5 (3): 32002–32012. doi:10.1063/1.3608133. PMC 3194786. PMID 22007266.