Escherichia coli O157:H7 is an emerging cause of food-borne illness. This bacterial pathogen, most commonly transmitted by undercooked ground beef, causes hemorrhagic colitis. It has been associated with the hemolytic uremic syndrome and death, mostly in children and the elderly. This article describes three patients treated for sporadic cases of Escherichia coli O157:H7-associated hemorrhagic colitis at Charleston Area Medical Center over a three-week period.