pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis

R R Adams; A A Tavares; A Salzberg; H J Bellen; D M Glover

doi:10.1101/gad.12.10.1483

pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis

Genes Dev. 1998 May 15;12(10):1483-94. doi: 10.1101/gad.12.10.1483.

Authors

R R Adams¹, A A Tavares, A Salzberg, H J Bellen, D M Glover

Affiliation

¹ Cancer Research Campaign (CRC) Laboratories, Cell Cycle Genetics Research Group, Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, UK. d.m.glover@dundee.ac.uk

Abstract

Mutations in the Drosophila gene pavarotti result in the formation of abnormally large cells in the embryonic nervous system. In mitotic cycle 16, cells of pav mutant embryos undergo normal anaphase but then develop an abnormal telophase spindle and fail to undertake cytokinesis. We show that the septin Peanut, actin, and the actin-associated protein Anillin, do not become correctly localized in pav mutants. pav encodes a kinesin-like protein, PAV-KLP, related to the mammalian MKLP-1. In cellularized embryos, the protein is localized to centrosomes early in mitosis, and to the midbody region of the spindle in late anaphase and telophase. We show that Polo kinase associates with PAV-KLP with which it shows an overlapping pattern of subcellular localization during the mitotic cycle and this distribution is disrupted in pav mutants. We suggest that PAV-KLP is required both to establish the structure of the telophase spindle to provide a framework for the assembly of the contractile ring, and to mobilize mitotic regulator proteins.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism
Amino Acid Sequence
Animals
Cell Division / genetics*
Cell Size
Centrosome / ultrastructure
Cloning, Molecular
Contractile Proteins / metabolism
Drosophila Proteins*
Drosophila melanogaster / cytology
Drosophila melanogaster / embryology
Drosophila melanogaster / genetics*
Embryo, Nonmammalian / ultrastructure
Genes, Insect*
Insect Proteins / genetics
Insect Proteins / metabolism
Insect Proteins / physiology*
Mammals / metabolism
Microfilament Proteins*
Microtubule-Associated Proteins / chemistry
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / physiology*
Molecular Sequence Data
Nervous System / cytology
Nervous System / embryology*
Protein Serine-Threonine Kinases / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Spindle Apparatus / ultrastructure*
Subcellular Fractions / chemistry
Telophase / genetics*

Substances

Actins
Contractile Proteins
Drosophila Proteins
Insect Proteins
Microfilament Proteins
Microtubule-Associated Proteins
anillin
pav protein, Drosophila
pnut protein, Drosophila
polo protein, Drosophila
Protein Serine-Threonine Kinases